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Epithalon does not appear to affect telomerase in transformed (cancer) cells, which already express constitutive telomerase activity, though this observation requires further investigation

Fluorescence detection occurred with excitation at 380 nm and emission at 480 nm

To terminate the cultures, 20 L of 0.1% Triton X-100 and 30 L of lyticase (1 mg/mL in 0.1 M phosphate buffer, pH 7.5, containing 50 mM mercaptoethanol) were added, and the plates were incubated for 1 h at 35C

Mol Genet Genomics 291, 16391646 (2016)

albicans , alone or in combination with azoles, potentially via vacuolar dysfunction and reduced extracellular phospholipase activity (Yousfi et al., 2019